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                                                                                                                                                   ;                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      #                                                                                  
         8   
                                                            	                                     
                                                                               -      	      
                        U   $         	         
         	            
            
                                          (      	                F   "      3   (      )   8   #   *   ±   _      %         )      z                                                                                                                                                                      ;                                                                                                                                                                                                                                                                                                                                                            #                              
   8 
                      	             
                             -  	  
        U $   	   
   	    
    
              (  	      F "  3 (  ) 8 # * ± _  %   )  z   |„  }ê  ~
  ~H  ƒŒ  ‰f  ‘.ü¢EP!ÏrÐºL	r	É	U
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Ë
,…ûú²É€;(FÜ�š0ë
N–…¬G‘6Ûãˆ(
hc)%ÿ<Å * ¤ ë T-%g¦g¦X  * ¤ Ä ë H`�½ÊöÿTw øLŠØ	á	â
16?n"é{µX Ó"(æ(T|£êóŒK~â
af§U ÂÅö�i/Z}ï�N€G€1€NG?(FÜ�¶ÎjšLÈ0‚>†�;y  S^ñOýÕM³ñOê“ê„çr	e
†˜r	>
�ÀoÍ9çtÅ#­ëÅ
Æ�Â
ñtÍ+DD‡/ZO „ × ‘&<�eð€à	Ïª„ Ob¾8 §OÃ{	
_
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ÆHcP‡c
Ue¥ søþ[
Œ²¶1ú8‰X©l¨¯°£Ëù ö.O „ × ‘&<_
âœvWlCê¡Á‰¾	&
³$Ê$Îh0¿‚Æ#]áðPÑÊÆÄË	
ó
#$
Œ8l«Æ#é£2K
àÊ—_
ß¶F]¥»ðÄ
NÕFÅ"¥ü Žéo$xó²2Ìv_
º
‡½‚WwlÞM §OiÆåÃÝ{	
_
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GÆH«A‰#$c/P®×ª‡ÓÃûýZ–ìToðÍó(B>*HÔw�ÆUª…	‡	|
ž
Ci#1^Ø=… #]§(œÀOæŠcðÆS”	¡¸KºçUp
 ÂëÆ+@ÖE´z‹j¡fÔ[};Ð	Ó	9Jþ²¶úÉàñFŽ±®Ÿ	ø<›‚»÷�[þÙ²¶úxò“;óÈ>*ªØ PÑÊ‰ÆÄË¾		
&
ó
³#é$Êñ–óQºê€>*ª	Ø ]§(ÁpÍèc* ë ¥Æö:Aðëïe©úC	æ	T
6Š+/@DKŽå>E~„´J]’¥TÎŒTXz‡‹œ*Vj}f£ùNò^>*ª‡	|
CØ s_C“’	]¥¤ê(M?ì«kvÌØõ–ø3 è�úô4Â06€”ßòæÐ."“š
Ðåïú‰2Ä°£ÓÃËûù ö.QH�d‚SôrÀÿ
yÐö4_§¿ s_Cð`� ïCn”s<—–	)_Â‚¾CE�7eÍsð$Ð€áÊU'ŸóQ/¥>¹¾í�›ÓY»pº›:ÔhN¨`ÞÞq‹ë+@E´z‹jfôV
”Æó_>3°vK"ïÿáV2ôð–ìýTZoðÍ¦Þ|rñAóŒÉ"’Œ Á$¯ ÿ  S×ñO–6rb¾8)�ù8¥›Ù‘Ž«·kÌõ  @í¬\!wÙ/ ð XÕE�Æ…“
ž
Þ
è“_‹>u†?�P´æ~‹ƒÉÊ7>qt‚	óö�þ7ã	Ž	H¨X{5Ò%ZËác�¨âõ6Ij‹�@�„R·3%�) ê Øi¼ÆÇæÝGÄ#$±M@m®×ýìHÖ³Ü#e»™B.{“u
E„	˜	Ç
›ÒÒÈ@¼æÄ@7>‚ó�ã	Òác�6j�„R·%Êqt	öþ7Ž	H¨X{5%ZË¨âõI‹�@3�TP^ŒçM	Õdþ¨$CJ-yfr¢ób¼
4g‰	¦
‡%ùÑFÐ„{
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  Ò<Notes>
<UUID>2e9fa067-f504-47cf-aeea-faf01f87f75b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGL4.29-Luc2P-CRE-Hygro-CMV-EGFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter and a cAMP response element for studying cell signaling using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2006.9.25</Created>
<LastModified UTC="5:46:34">2020.9.30</LastModified>
<AccessionNumber>DQ904461</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Mammalian Cells</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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<UUID>77e481f5-1a7f-4e68-a03a-b7bb7798bc92</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGL4.29[luc2P/CRE/Hygro]</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter and a cAMP response element for studying cell signaling using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2006.9.25</Created>
<LastModified UTC="9:48:17">2020.9.29</LastModified>
<AccessionNumber>DQ904461</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Mammalian Cells</Organism>
<SequenceClass>UNA</SequenceClass>
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  ‹     ÿ BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;hygromycin B phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV*"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7251" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6460-7251" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7252-7320" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5672-6260" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3848-4205" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3533-3654" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="hPEST" directionality="1" translationMW="4153.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3484" maxFusedRunOn="3484"><Segment range="3362-3481" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;PEST degradation sequence from mouse ornithine decarboxylase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"/></Q></Feature><Feature recentID="13" name="pause site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7487-7578" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase II transcriptional pause signal from the human&amp;nbsp;Î±2 globin gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5297-5345" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7425-7473" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="minP" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1645-1676" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="15" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  Ü<Notes>
<UUID>202246de-adba-4390-b458-44f9cbde5b52</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGL4.29-CRE-Luc2P-Luc-SV40-Hygro-CMV-EGFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter and a cAMP response element for studying cell signaling using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2006.9.25</Created>
<LastModified UTC="20:59:55">2025.8.28</LastModified>
<AccessionNumber>DQ904461</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Mammalian Cells</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y      K         
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  «<?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="1" name="Unique Cutters + BglI, SfiI" enzymeNames="AarI Acc65I AlwNI ApaLI AvrII BamHI BbvCI BciVI BfuAI BglI BglII BlpI BmtI BpmI BsaAI BsmBI BspMI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I DraIII Eco53kI EcoRI FseI HindIII KasI KpnI MfeI MreI NarI NheI NotI PciI PluTI PmeI PsiI PstI PvuI SacI SacII SalI SfiI SfoI SgrAI SpeI StuI XmnI"/></CustomEnzymeSets>
